Dulbecco’s Phosphate Buffered Saline from MilliporeSigma

Product Specs
ItemDulbecco’s Phosphate Buffered Saline
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberD5773
Applicationsmammalian cell culture
Quantity10L, 10X1L, 25KG, 50KG, 50L

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Controlled Level of Contamination Coupled to Deep Sequencing (CoLoC-seq) Probes the Global Localisation Topology of Organelle TranscriptomesBIO-PROTOCOLSeptember 20, 2023Anna Smirnova, Damien Jeandard, Alexandre Smirnovcatalog number: S4117)Dulbecco’s phosphate buffered saline (DPBS 1×) (Sigma-Aldrich, catalog number: D5773)20% sodium dodecyl sulfate (SDS) (Euromedex, catalog number: EU-0660-B)n-dodecyl-β-D-maltoside (Sigma-Aldrich,

(2) Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditionsJournal of Biological ChemistryMay 1, 2023Victoria Karakis, Mahe Jabeen, John W. Britt, Abigail Cordiner, Adam Mischler, Feng Li, Adriana San Miguel, Balaji M. Raoparaformaldehyde in PBS,Thermo Fisher Scientific,Cat#R37814 Triton X-100,Sigma,Cat#T8787 PBS w/o Ca/Mg,Sigma,Cat#D5773 PBS w/Ca/Mg,Sigma,Cat#D8662 Human IgG,Immunoreagents,Cat#Hu-003-C BSA,Thermo Fisher Scientific,Cat#BP9703 10%

(3) Cell-Friendly Chitosan-Xanthan Gum Membranes Incorporating Hydroxyapatite Designed for Periodontal Tissue RegenerationPharmaceuticsFebruary 20, 2023Rafael Maza Barbosa, Daniel Navarro da Rocha, Renata Francielle Bombaldi de Souza, Jheison Lopes Santos, José Ricardo M. Ferreira, Ângela Maria Moraesperformed in all membrane formulations after immersion in phosphate-buffered saline (PBS, product reference D5773, Sigma–Aldrich, St. Louis, MO, USA) solution and incubation for 1 h at 35 °C. Topographic images and

(4) Low oxygen tension potentiates proliferation and stemness but not multilineage differentiation of caprine male germline stem cellsMolecular Biology ReportsJune 1, 2021Shiva Pratap Singh, Suresh Dinkar Kharche, Manisha Pathak, Ravi Ranjan, Yogesh Kumar Soni, Manoj Kumar Singh, Ramasamy Pourouchottamane, Manmohan Singh ChauhanInstitute. After 3 to 4 washings with Dulbecco’s phosphate-buffered saline (DPBS; Sigma-Aldrich, Cat#D5773) and removal of connective tissue, testes were processed for collection and enrichment of cmGSCs. Isolation of

(5) Two distinct trophectoderm lineage stem cells from human pluripotent stem cellsThe Journal of biological chemistryJanuary 1, 2021Mischler A, Karakis V, Mahinthakumar J, Carberry CK, San Miguel A, Rager JE, Fry RC, Rao BM.Paraformaldehyde in PBS,Thermo Fisher Scientific,Cat#R37814 Triton X-100,Sigma,Cat#T8787 PBS w/o CaMg,Sigma,Cat#D5773 PBS w/CaMg,Sigma,Cat#D8662 Human IgG,Immunoreagents,Cat#Hu-003-C BSA,Fisher Scientific,Cat#BP9703 10%

(6) An EdU-based flow cytometry assay to evaluate chicken T lymphocyte proliferationBMC Veterinary ResearchDecember 1, 2020Karla Lucía F. Alvarez, Astrid Poma-Acevedo, Manolo Fernández-Sánchez, Manolo Fernández-Díazpelleted by centrifugation for 5 min at 300 ×g and resuspended in 4 ml of D-PBS (Sigma Aldrich, Catalog # D5773-50 L). Mononuclear cells were isolated by density gradient centrifugation for 30 min at 400 ×g using

(7) A transient increase in MHC-IIlow monocytes after experimental infection with Avibacterium paragallinarum (serovar B-1) in SPF chickensVeterinary ResearchDecember 1, 2020Karla Lucía F. Alvarez, Astrid Poma-Acevedo, Manolo Fernández-Díazsubsequently transported to the laboratory in ice-cold D-PBS (Sigma-Aldrich, St. Louis, MO, USA, Cat. no. D5773-50L). Genomic DNA was extracted using a commercial QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany,

(8) Capturing, induced spawning, and first feeding of wild-caught Pseudopimelodus mangurus, an endangered catfish speciesLatin American Journal of Aquatic ResearchJuly 6, 2020Dilberto Ribeiro Arashiro, George Shigueki Yasui, Leonardo Luiz Calado, Nivaldo Ferreira Do Nascimento, Silvio Carlos Alves do Santos, Gustavo Fonseca Shiguemoto, Paulo Sérgio Monzani, José Augusto Senhoriniwas measured, and a small aliquot was weighed and fixed in 2% glutaraldehyde in Dulbecco's PBS (Sigma #D5773, St. Louis, USA) in order to calculate the number of oocytes per gram, total fecundity (total number

(9) Autologous Adipose-Derived Stem Cells, Platelet-Rich Plasma, and Fibrin Enhance Healing of Mandibular Bone Defects in SwineInternational Journal of Regenerative MedicineJune 18, 2020Matthew B. Wheeler, Aaron J. Maki, Anna C. Ercolin, Jamey J. Cooper, Kelly S. Roballo, Marcello Rubessa, Matthew B. Wheeler, Rathnaweera A.C. Rabelbuffer (Sigma R7757, St. Louis, MO) was added to the pellet for 2 minutes, then diluted with PBS (Sigma D5773) and centrifuged for 5 minutes at 1400 rpm (547 X g). The isolation of ASCs was repeated 1-2 times to

(10) The Effects of Vitamin D3, Vitamin B12, and Vitamin K on the Differentiation of Porcine Adipose-Derived Stem Cells (ASC) into OsteoblastsJournal of Biology Engineering and MedicineMay 11, 2020Matthew B. Wheeler, Bane T.A., Bertels J.C., Matthew B. Wheeler, Polkoff K.M., Rubessa M.Strips of sterile fat were washed twice in Dulbecco’s Phosphate Buffer Saline (DPBS, Sigma Aldrich D5773, St. Louis, MO) containing 1% Penicillin GStreptomycin (Sigma P3539) and 5.0 mg/L of Amphotericin B

(11) A reversible autophagy inhibitor blocks autophagosome-lysosome fusion by preventing Stx17 loading onto autophagosomesMolecular biology of the cellAugust 1, 2019Vats S, Manjithaya R.(Life Technologies; 15140-122) at 5% CO2 and 37°C. Autophagy was induced by washing cells with DPBS (Sigma-Aldrich; D5773) and incubating them in starvation media/Earle’s balanced salt solution (Sigma-Aldrich; E7510) made

(12) Synchronizing developmental stages in Neotropical catfishes for application in germ cell transplantationZygoteApril 1, 2018Dilberto Ribeiro Arashiro, George Shigueki Yasui, Leonardo Luiz Calado, Nivaldo Ferreira do Nascimento, Matheus Pereira dos Santos, Silvio Carlos Alves do Santos, Nycolas Levy-Pereira, Paulo Sérgio Monzani, Diógenes Henrique Siqueira-Silva, José Augusto Senhoriniwas collected randomly and fixed in 2.5% glutaraldehyde in Dulbecco’s phosphate-buffered saline (D-PBS) (Sigma #D5773, St. Louis, USA). The samples were collected over the following time intervals: every 2 min postfertilization

(13) Short-term storage of the oocytes affects the ploidy status in the yellowtail tetra Astyanax altiparanaeZygoteFebruary 1, 2018Matheus Pereira dos Santos, Nivaldo Ferreira do Nascimento, George Shigueki Yasui, Nycolas Levy Pereira, Takafumi Fujimoto, José Augusto Senhorini, Laura Satiko Okada Nakaghiwere then immersed in calcium and magnesium-free Dulbecco’s phosphate-buffered saline (dPBS) (Sigma #D5773), enriched with 0.5% bovine serum albumin (BSA) and maintained in an incubator with oxygen demand and temperature

(14) FLI1 and PKC co-activation promote highly efficient differentiation of human embryonic stem cells into endothelial-like cellsCell death & diseaseJanuary 26, 2018Zhao H, Zhao Y, Li Z, Ouyang Q, Sun Y, Zhou D, Xie P, Zeng S, Dong L, Wen H, et al.

(15) Demonstration of parallel algal processing: production of renewable diesel blendstock and a high-value chemical intermediateGreen ChemistryJanuary 1, 2018Eric P. Knoshaug et al.and assignments of compounds identified. Cloud points of HI products were determined by ASTM method D5773. Distillation temperatures of HI products were determined by GC-FID based simulated distillation following ASTM

(16) A Flow Cytometry Protocol to Estimate DNA Content in the Yellowtail Tetra Astyanax altiparanaeFrontiers in GeneticsSeptember 25, 2017Pedro L. P. Xavier, José A. Senhorini, Matheus Pereira-Santos, Takafumi Fujimoto, Eduardo Shimoda, Luciano A. Silva, Silvio A. dos Santos, George S. Yasuioccasional mixing. Staining was achieved by addition 800 μL of Calcium-Free Dulbecco's PBS (Sigma #D5773) containing DAPI at 1 μg mL−1. The samples were then filtered by a 30-μm mesh (Celltrics, Partec GmBH,

(17) Discovery of pan autophagy inhibitors through a high-throughput screen highlights macroautophagy as an evolutionarily conserved process across 3 eukaryotic kingdomsAutophagySeptember 2, 2017Mishra P, Dauphinee AN, Ward C, Sarkar S, Gunawardena AHLAN, Manjithaya R.following appropriate treatments, cells were washed with ice cold phosphate-buffered saline (PBS; Sigma, D5773). Cells were then lysed in 100 μl of sample buffer (10% [w:v] SDS, 10 mM DTT, 20% [v:v] glycerol, 0.2

(18) Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cellsNature ProtocolsSeptember 1, 2017Jeffrey D Martell, Thomas J Deerinck, Stephanie S Lam, Mark H Ellisman, Alice Y Tingequipment while working with Durcupan ACM components. Dulbecco’s PBS (DPBS; Sigma-Aldrich, cat. no. D5773) BSA (Thermo Fisher Scientific, cat. no. BP1600) Methanol (Sigma-Aldrich cat. no. 34966) ! CAUTION

(19) Chordotonal organs in hemipteran insects: unique peripheral structures but conserved central organization revealed by comparative neuroanatomyCell and Tissue ResearchDecember 1, 2016Hiroshi Nishino, Hiromi Mukai, Takuma Takanashiperipheral nerves of interest were exposed and filled with Dulbecco’s phosphate buffered saline (DPBS; D5773, Sigma Aldrich). The nerve was cut with microscissors (Vananas Scissors, 501778, WPI, FL, USA) and the

(20) Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development ofAstyanax altiparanae(Teleostei, Characidae)ZygoteDecember 1, 2016Matheus Pereira dos Santos, George Shigueki Yasui, Pedro Luiz Porfírio Xavier, Nadya Soares de Macedo Adamov, Nivaldo Ferreira do Nascimento, Takafumi Fujimoto, José Augusto Senhorini, Laura Satiko Okada Nakaghi2-min intervals and fixed in 2% glutaraldehyde in Dulbecco’s phosphate-buffered saline (PBS) (Sigma #D5773) until 30 mpf. For observation of the male and female pronucleus, the eggs were washed in dPBS and stained

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Dulbecco’s Phosphate Buffered Saline from MilliporeSigma

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